Widely cited, comprehensive and flexible profiling assay to characterise novel DUB targeting compounds to establish their potency and selectivity
DUBprofiler™ is based on a robust ubiquitin-rhodamine(110)-glycine substrate based-assay, comprising a panel of DUB enzymes representative of the entire human DUB family. DUB targeting compounds can be rapidly screened to establish their potency and selectivity.
The assay is highly scalable and flexible, allowing from 1 to 1000s of compounds to be screened at any combination of DUBs on the panel. Compounds can be screened at either single concentrations or across standard 8-point half-log concentration-effect curves where IC50 determinations are required.