- Catalogue Number
- 64-0053-050
- Product Size
- 50 µg
- Price £
- £430
- Accession Number
- NP_005145
- Residues Expressed
- 1-1118
- Certificate of Analysis Size
- 50 µg
- Species
- human
- Source
- E.coli
- Quantity
- 50 µg
- Storage
- -70°C
- Concentration
- 0.5 mg/ml
- Formulation
- 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
- Molecular Weight
- ~128kDa
- Stability
- 12 months at -70°C; aliquot as required
- Protein Sequence
- Accession number: NP_005145. For full protein sequence information download the Certificate of Analysis pdf.
- QA; Protein Identification
- Confirmed by mass spectrometry.
- QA Activity
Deubiquitylase Enzyme Assay: The activity of USP8 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of USP8 was compared confirming the deubiquitylating activity of USP8.